Why 660 g/mol?
The average molecular weight of a double-stranded DNA base pair is approximately 660 Daltons (g/mol).
Calculator
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Results
Number of Copies
1.82e+10
Copy Number Analysis
Understanding DNA quantification
Molecular Calculation
Your DNA sample contains approximately 1.82e+10 copies. This calculation is essential for PCR, cloning, and sequencing applications.
Applications
- • PCR template dilution
- • Cloning efficiency calculations
- • Next-generation sequencing library prep
- • qPCR standard curve preparation
How to Use
Step-by-step instructions
- 1Enter the amount of DNA in nanograms (ng).
- 2Enter the length of the DNA template in base pairs (bp).
- 3The calculator will display the estimated number of copies.
Copy Number Formula
Calculates the number of molecules (copies) based on the mass of DNA and its length. It assumes the average molecular weight of a base pair is 660 g/mol.
Copies = (Amount × 6.022×10²³) / (Length × 1×10⁹ × 660)Variables:
AmountMass of DNA in nanograms (ng)LengthLength of DNA in base pairs (bp)6.022×10²³Avogadro's numberExample
Plasmid Sample
Inputs:
Amount:100 ng
Length:5000 bp
Steps:
- 1.Numerator: 100 × 6.022×10²³ = 6.022×10²⁵
- 2.Denominator: 5000 × 10⁹ × 660 = 3.3×10¹⁵
- 3.Result: 1.82×10¹⁰ copies
Result:
1.82e+10